ISOGEN-LS

Manufacturer :: Nippon Gene Co., Ltd.

Storage Condition :: Keep at 2-10 degrees C. (RT)

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 317-02623 Manufacturer 317-02623 Barcode No 4987481583238	10mL	List Price JPY 13,000	In stock in Japan
	Distributor 311-02621 Manufacturer 311-02621 Barcode No 4987481516281	100mL	List Price JPY 37,000	In stock in Japan

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Overview / Applications

Outline	This product is for research use only. Do not administer it to human. ISOGEN and ISOGEN-LS both employ an extraction procedure for total RNA purification from a wide range of samples of human, animal, plant and bacterial origin. ISOGEN proves useful for samples with small quantity availability, because it provides a high relative yield and high purity of intact RNA. Meanwhile, time involvement is minimal, as a somewhat laborious CsCl gradient need not be incorporated, attributable to this ISOGEN procedure. ISOGEN is suitable for samples such as tissues and culture cells. ISOGEN-LS is suitable for high volume samples, examples of which include blood and fluid samples which are difficult to concentrate. Characteristics: [1] ISOGEN and ISOGEN-LS are homogenous solutions, consisting mainly of phenol and guanidinium thiocyanate. [2] The extraction procedures which are employed here, induce a liquid phase separation, resulting in a conveniently tinted aqueous RNA layer. [ISOGEN: blue, ISOGEN-LS: red] [3] Through employment of the provided protocol, RNA, DNA, and protein are separated from one another in one series of steps. [4] The ISOGEN procedures are easy and quick. Each single separation provides -RNA in approximately 1 hour, -DNA in a little over 2 hours, -and protein extraction in a little over 2 and a half hours. [5] High Recovery Rate

Outline

This product is for research use only. Do not administer it to human.

ISOGEN and ISOGEN-LS both employ an extraction procedure for total RNA
purification from a wide range of samples of human, animal, plant and bacterial
origin. ISOGEN proves useful for samples with small quantity availability,
because it provides a high relative yield and high purity of intact RNA.
Meanwhile, time involvement is minimal, as a somewhat laborious CsCl gradient
need not be incorporated, attributable to this ISOGEN procedure.

ISOGEN is suitable for samples such as tissues and culture cells.
ISOGEN-LS is suitable for high volume samples, examples of which include
blood and fluid samples which are difficult to concentrate.

Characteristics:

[1] ISOGEN and ISOGEN-LS are homogenous solutions, consisting
mainly of phenol and guanidinium thiocyanate.
[2] The extraction procedures which are employed here, induce
a liquid phase separation, resulting in a conveniently tinted aqueous RNA
layer. [ISOGEN: blue, ISOGEN-LS: red]
[3] Through employment of the provided protocol, RNA, DNA, and
protein are separated from one another in one series of steps.
[4] The ISOGEN procedures are easy and quick. Each single
separation provides
-RNA in approximately 1 hour,
-DNA in a little over 2 hours,
-and protein extraction in a little
over 2 and a half hours.
[5] High Recovery Rate

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Manufacturer Information

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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