Cycloamylose

Cycloamylose is a mixture of high molecular weight cyclic alpha-1,4-glucan produced by the action of 4-alpha-glucanotransferase on starch ⁽¹⁾. Cycloamylose has a single helical V-amylose conformation, with an hydrophobic channel-like cavity ⁽²⁾ and can form an inclusion complex with various guest molecules.

Features:

1. Cycloamylose is easily degraded by alpha-amylases. Upon storage and handling of cycloamylose, cares should be taken to avoid the contamination of alpha-amylases either from human saliva or from microorganisms.


2. Cycloamylose powder can be stored at room temperature.


3. Cycloamylose can be dissolved in water to make up to 30% (w/v) solution. It is highly recommended to use autoclaved distilled water, so that to avoid a contamination of alpha-amylases. The cycloamylose solution is recommended to store at -20 degrees C.

1. "The functions of 4-alpha-glucanotransferases and their use for the production of cyclic glucans.", T. Takaha and S. M. Smith, Biotechnol. Genet. Eng. Rev., 16, 257-280 (1999).


2. "V-amylose at atomic resolution: X-ray structure of a cycloamylose with 26 glucose residues (cyclomaltohexaicosaose).", K. Gessler, I. Uson, T. Takaha, N. Krauss, S. M. Smith, S. Okada, G. M. Sheldrick, and W. Saenger, Proc. Natl. Acad. Sci. USA, 96, 4246-4251 (1999).


3. "Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose", Terada Y., Fujii K., Takaha T. and Okada S., Appl. Environ. Microbiol., 65(3), 910-915 (1999)


4. S. Kitamura, et al., Carbohydr. Res., 304, 303-314 (1997)


5. "X-ray structure of the cyclomaltohexaicosaose triiodide inclusion complex provides a model for amylose-iodine at atomic resolution", Nimz O., Gessler K., Usón I., Laettig S., Welfle H., Sheldrick GM, Saenger W., Carbohydr. Res., 338(9), 977-986 (2003)


6. "Inclusion complexes of V-amylose with undecanoic acid and dodecanol at atomic resolution: X-ray structures with cycloamylose containing 26 D-glucoses (cyclohexaicosaose) as host", Nimz O., Gessler K., Usón I., Sheldrick GM, Saenger W., Carbohydr. Res., 339(8), 1427-1437 (2004)


7. S. Kitamura, et al., J. Appl. Glycosci., 50, 321-325 (2003)


8. S. Kitamura and Y. Terada, "Fiber Super-biomimetics", NTS, p141.


9. C. Urakami, et al., PACIFICHEM 2005