For extracting mitochondrial DNA from tissues, cells, and compound feeds
mtDNA Extractor® CT Kit
This product is a kit for extracting mitochondrial DNA (mtDNA) from mammalian tissues and cells, quickly and easily. It can also be used to extract mitochondrial DNA from compound feeds.
Features
- mtDNA detected by agarose gel electrophoresis can be extracted from 50 to 250 mg of tissue.
- The extracted mtDNA can be used in PCR and restriction enzyme digestion.
- mtDNA can also be extracted from frozen tissues.
- Organic solvents such as phenol or chloroform are not required.
Principle
- Cell lysate is centrifuged to obtain the mitochondrial fraction.
- Genomic DNA in the mitochondrial fraction is removed by alkaline denaturation and precipitation using the alkaline-SDS method, which is used for plasmid DNA purification, and mitochondrial DNA, which is circular DNA, is recovered.
- Contaminating proteins are solubilized with sodium iodide, and mitochondrial DNA is purified with Washing Solution.
Procedure
Example of experiment
Extraction of mitochondrial DNA from fresh and frozen mouse tissues
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Half of the mtDNA extracted from fresh mouse tissues was digested with Pst I and confirmed by agarose gel electrophoresis. -
Half of the mtDNA extracted from frozen mouse tissues was digested with Pst I and confirmed by agarose gel electrophoresis.
Precautions
Since this kit is designed to extract small amounts of mitochondrial DNA, we recommend GelRed™ staining to detect mitochondrial DNA.The mitochondrial DNA cannot be detected by ethidium bromide staining.
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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